利用mrna差异显示技术克隆恶疫霉侵染诱导-南京农业大学学报.pdfVIP

摇 南京农业大学学报摇 2012,35(4):54-58 摇 Journal of NanjingAgricultural University http:/ / 試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試 陈孝仁,张博月,May Bente Brurberg,等.利用 mRNA 差异显示技术克隆恶疫霉侵染诱导表达的草莓基因[J].南京农业大学学报,2012, 35(4):54-58 利用mRNA差异显示技术克隆恶疫霉侵染 诱导表达的草莓基因 1,2* 1 rberg2 陈孝仁 ,张博月 ,May Bente Bru (1.扬州大学园艺与植物保护学院,江苏 扬州225009;2.Plant Health and Plant Protection Division,Norwegian Institute for Agricultural and Environmental Research(Bioforsk),H覬gskoleveien7,魡s 1432,Norway) 摘要:为了解草莓与恶疫霉互作的分子机制,以接种后3、5和7 d 的感病草莓品种‘FDP821爷的冠部为材料,利用mRNA差 异显示技术研究‘FDP821爷与恶疫霉亲和互作前后基因表达的差异,获得78条差异表达的cDNA片段,通过分析从中分离 出恶疫霉侵染诱导表达的10个草莓基因cDNA片段。 BLASTX在线分析表明,这些草莓基因的功能涉及呼吸作用、衰老和 核苷运输与代谢等。 为阐明草莓与恶疫霉互作的分子机制提供了重要的线索。 关键词:草莓冠腐病;恶疫霉;mRNA差异显示技术 中图分类号:S668.4摇 摇 摇 摇 文献标志码:A摇 摇 摇 摇 文章编号:1000-2030(2012)04-0054-05 Cloning of strawberry genes induced by Phytophthora cactorum infection using mRNA differential display RT鄄PCR 1,2* 1 2 CHEN Xiao鄄ren ,ZHANG Bo鄄yue ,May Bente Brurberg (1.College of Horticulture and Plant Protection,Yangzhou University,Yangzhou225009,China;2.Plant Health and Plant Protection Division,Norwegian Institutefor Agricultural and Environmental Research(Bioforsk),H覬gskoleveien7,魡s 1432,Norway) Abstract:To explore the molecular mechanisms underlying the interaction of strawberry with Phytophthora cactorum,the strawberry variety ‘FDP821爷susceptible toP.cactorumwasemployedinthe study andthediseasedcrowntissueswereharvestedat3,5 and7 days after inoculation.The differentially expressed genes during the compatible interaction between strawberry