气相色谱-质谱法测定蜂王浆中的植物甾醇组成.pdfVIP

※分析检测 食品科学 2010, Vol. 31, No. 18 317 1,2 1,2, 1,2 1 1 1 徐 响 ，孙丽萍 * ，董 捷 ，李天骄 ，穆雪峰 ，沈新锋 (1. 中国农业科学院蜜蜂研究所，北京 100093 ；2. 国家农产品加工技术研发中心蜂产品分中心，北京 100093) 为探明蜂王浆中的植物甾醇组成，蜂王浆样品经冻干、萃取得到王浆脂类物质，再经过皂化、衍生后 使用 HP-5MS 柱进行分离，采用 GC-MS 对蜂王浆中甾醇类物质进行鉴定，并用 GC-FID 内标法进行定量分析。结 果表明：蜂王浆中除含有少量胆固醇外，还含有 25- 羟基 -24- 甲基胆甾醇、菜油甾醇、豆甾醇、β- 谷甾醇、Δ5- 燕麦甾醇、Δ7- 燕麦甾醇等植物甾醇；蜂王浆中总甾醇含量在 51mg/100g 鲜王浆以上。其中，25- 羟基 -24- 甲基胆 甾醇、菜油甾醇、β- 谷甾醇和Δ5- 燕麦甾醇含量较高，分别占总甾醇含量的 36.79% 、6.30% 、19.75% 和 27.99% 。 蜂王浆；植物甾醇；气相色谱 - 质谱法 Sterol Composition Analysis of Royal Jelly by Gas Chromatography Coupled with Mass Spectrometry 1,2 1,2, 1,2 1 1 1 XU Xiang ，SUN Li-ping *，DONG Jie ，LI Tian-jiao ，MU Xue-feng ，SHEN Xin-feng (1. Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China； 2. Bee Product Center, National RD Center for Agro-Food Processing, Beijing 100093, China) Abstract：To figure out the sterol composition of royal jelly, royal jelly samples were lyophilized and extracted and the extracted lipid substances were then saponified, derivatized and separated on a HP-5MS column prior to gas chromatography with flame ionization detection (GC-FID ) coupled with mass spectrometric identification of sterols, and quantitative analysis was finally conducted by internal standard method. A small amount of cholesterols was contained in royal jelly, and the main sterols were 25-hydroxy-24-methylcholesterol, campesterol, stigmasterol, β-sitosterol, Δ5-avenasterol and Δ7-avenasterol. The total content of sterols in 100 g of fresh royal jelly was higher than 51 mg. Among these sterols, the most predominant compone