关于的毕业论文化学发光免疫分析.doc

　　关于的毕业论文化学发光免疫分析 关于的毕业论文化学发光免疫分析 导读：tionofanalysisandstatisticalmethodology.Theresultsshog/L, 酶标蛋白A稀释比例为1：2000，温育时间30分钟时信噪比最大为1284，数倍高于其它组合。方法学评价表明该实验条件下检测灵敏度为20ng/L；其线性范围为 20-20000ng/L；精密度良好，平均批内CV为5.32%，平均批间CV为8.82%。实验结果证实该方法可用于anti-ALB McAb的克隆筛选，且方法学上明显优于ELISA等传统方法，特别是一步操作的简便快速，也适合其它抗体高通量筛选。 关键词：化学发光免疫分析、抗人白蛋白单克隆抗体、正交试验、克隆筛选 Chemiluminescence immune assay for screening and cloning of anti human albumin Monoclonal Antibody Abstract: the chemiluminescent immunoassay for monoclonal antibody of mouse anti human albumin (anti-ALB McAb) cloning and screening. Simplified method for one-step operation, through the orthogonal experiment [L9 (34) surface] optimization of experimental parameters, find the positive clone/blank control the light value(SNR) parameters of maximum and the bination of analysis and statistical methodology. The results showed that the coating anti 3 4 5 6 7 8 9 10 关于的毕业论文化学发光免疫分析 导读：fotherbination.Theevaluationshoentalconditionsforthe20ng/Lmethod;thelinearrangeis20-20000ng/L;goodprecision,theaveragenumberofCVberofCVis8.82%.Theexperimentresultsprovethatthismethodcanbeus body to 3mg/L, the enzyme labeled protein A dilution ratio e of 30minutes es higher than that of other bination. The evaluation shoental conditions for the 20ng/L method; the linear range is 20-20000ng/L; good precision, the average number of CV ber of CV is 8.82%. The experiment results prove that this method can be used for screening and cloning of the anti-ALB McAb, and the method is obviously better than the ELISA and other traditional methods, especially the step of operation is simple and fast, but also for other antibody screening. Keyiluminescence immune analysis; screening of anti human albumin monoclonal antibody; clone; orthogonal test 2 目录 1引言 ................................................................................................................................................................... 1 1.1理论依据 .................................................