显微注射构建烟粉虱的RNAi技术体系-应用昆虫学报.PDFVIP

Chinese Journal of Applied Entomology 2015, 52(2): 504509. DOI: 10.7679/j.issn.20951353.2015.058 显微注射构建烟粉虱的RNAi 技术体系* ** *** 李如美 王少丽 张友军 100081 摘 要 RNAi RNAi dsRNA Bemisia tabaci RNAi CYP6CM1 dsRNA 4 PCR 50 nL dsRNA CYP6CM1 80%RNAi RNAi 关键词 RNA dsRNAPCR Establishment of a microinjection based RNA interference technique for Bemisia tabaci (Hemiptera: Aleyrodidae) ** *** LI Ru-Mei WANG Shao-Li ZHANG You-Jun (Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China) Abstract RNAi is a powerful tool for revealing gene function. Microinjection is the most widely-used method to obtain systematic interference by injecting dsRNA into insects. However, it’s very difficult to inject small insects, especially the sweetpotato whitefly, Bemisia tabaci . In order to verify the large-scale gene function of B. tabaci , it’s necessary to develop an RNAi technique. We established a method to silence the B. tabaci CYP6CM1 gene, the effect of which was detected by injecting the 4th instar larvae of whiteflies with different dsRNA concentrations. The results show that injection with different concentrations of dsRNA had various silencing effects on the CYP6CM1 gene, with a maximum silencing efficiency of more than 80%. This successful RNAi technology successfully can be used on other whiteflies. Key words RNA interference, microinjection, Bemisia tabaci , double strand rna, quantitative Real-time PCR RNAi