过度训练雌性大鼠骨密度的变化及其机制-中国康复医学杂志.PDFVIP

2011年，第26卷，第6期 ·基础研究· 过度训练雌性大鼠骨密度的变化及其机制* 张崇林 郑 陆1 2,3 摘要 目的：建立过度训练大鼠模型，并研究其骨量变化及机制。 方法：将225只3月龄雌性SD大鼠随机分为运动组120只和对照组105只。运动组和对照组大鼠各随机分成7组。 运动组大鼠进行17周的跑台训练。动态观察体重、血红蛋白（Hb）、血清雌二醇（E2）变化作为造模监控指标；检测 骨密度(BMD)、血清骨I型胶原氨基端延长肽(PINP)、骨I型胶原羧基末端肽交联(ICTP) 、基质金属蛋白酶8(MMP-8) 含量的变化，探讨骨量变化及其机制。 结果：在持续17周的跑台训练中，体重、Hb、E2显著性降低，符合过度训练的表现；BMD降低，血清PINP变化不明 显，血清ICTP显著性升高，血清MMP-8显著性升高。 结论：通过17周的跑台训练成功建立了过度训练动物模型；雌激素水平下降，导致MMP-8升高，骨分解代谢增强， 是过度训练导致低骨量的原因。 关键词 骨密度；过度训练；骨代谢；骨量；血清骨I型胶原氨基端延长肽；骨I型胶原羧基末端肽交联；基质金属蛋 白酶8 中图分类号:R493，R681 文献标识码：A 文章编号：1001-1242（2011）-06-0555-06 Research on the changes and its mechanism of bone mass density of overtraining female rats/ZHANG Chonglin,ZHENG Lu//Chinese Journal of Rehabilitation Medicine, 2011, 26(6): 555—560 Abstract Objective: To establish the model of overtraining female rat and explore the changes and its mechanism of bone mass. Method: A total of 225 aged 3-month female SD rats were randomly divided into training and control teams; training team of 120 rats were divided into 7 groups, the other 105 rats were divided into 7 groups also as con- trol team. The training groups were trained on rat-treadmill for 17 weeks. The body weight, concentration of he- moglobin (Hb) and estrogen (E2) in serum were observed dymamically as modeling indicators; the changes of bone mass density (BMD) and concentration of precollege type I amino terminal propertied (PINP), cross-linked c-telopeptide of collagen type I(ICTP) and matrix metalloproteinase 8(MMP-8) in serum were detected to probe thecourseofchangesofbone mass anditsmechanism. Result: After 17-weeks training period, the training groups had s