斑马鱼及的性腺发育.pptVIP

Composition : Ingredients mg/L INORGANIC SALTS Calcium chloride dihydrate 185.000 Magnesium chloride hexahydrate 200.000 Magnesium sulphate anhydrous 97.720 Potassium chloride 400.000 Potassium phosphate, monobasic 60.000 Sodium chloride 8000.000 Sodium phosphate, dibasic anhydrous 190.120 AMINO ACIDS DL-Alpha alanine 450.000 Glycine 200.000 L-Arginine (free base) 500.000 L-Asparagine 250.000 L-Cysteine (free base) 120.000 L-Glutamine 300.000 L-Histidine (free base) 250.000 L-Isoleucine 250.000 L-Leucine 125.000 L-Lysine hydrochloride 94.000 L-Methionine 75.000 L-Phenylalanine 125.000 L-Serine 200.000 L-Threonine 300.000 L-Tryptophan 20.000 L-Tyrosine Disodium Salt 276.160 L-Valine 100.000 VITAMINS Choline chloride 1.000 D-Ca-Pantothenate 1.000 Folic acid 1.000 Nicotinamide 1.000 Pyridoxine hydrochloride 1.000 Riboflavin-5-phosphate, Na 0.100 Thiamine monophosphate 1.000 i-Inositol 2.000 OTHERS D-Galactose 900.000 Phenol red Sodium Salt 11.000 Sodium pyruvate 550.000 Directions : 1. Suspend 14.0gms in 900ml tissue culture grade water with constant, gentle stirring until the powder is completely dissolved. Do not heat the water. Note: Presence of slight haziness in the medium is due to inherent nature of some of the ingredients in the composition. However, this will not affect the performance of the medium. 2. Adjust the pH to 0.2-0.3 pH units below the desired pH using 1N HCl or 1N NaOH since the pH tends to rise during filtration. 3. Make up the final volume to 1000ml with tissue culture grade water. 4. Sterilize the medium immediately by filtering through a sterile membrane filter with a porosity of 0.22 micron or less, using positive pressure rather than vacuum to minimize the loss of carbon dioxide. 交配中 公鱼在交配的过程中，采绝对主动方式，会先以眼睛观察并决定要向哪一只母鱼进行攻势。其次会进行求偶舞蹈，在想要交配的母鱼周围绕圈圈或是８字型，游向母鱼后，利用腹部侧面摩擦母鱼，刺激母鱼排卵，公鱼便会同时射精。 产卵 母鱼会一次把全部腹中的卵包括卵囊全部排出，一胎约会生出200-500颗卵。排出后母鱼会重新产生新的卵，约3-5天就可以再生下一次卵。 伴随着卵的释放，会有其他激素一起排出，这种激素可以让母鱼顺利把卵排放出来，另外一个功能则是让其他还产生卵的母鱼，停止排卵的行为，这在演化上算是一种竞争的结果。 注意事项（1）