阿魏菇多糖的结构分析.pdfVIP

现代食品科技 Modern Food Science and Technology 2015, Vol.31, No.3 阿魏菇多糖的结构分析  陈帅，许程剑，李应彪，刘冰，王雪铭，何燕  (石河子大学食品学院，新疆石河子 832000) 摘要：本文以阿魏菇中分离纯化得到的多糖-阿魏菇多糖(Pleurotus ferulae Lenzi polysaccharide, PFLP)作为研究对象，通过高效凝 胶过滤色谱来测定其分子量以及分布，通过气相色谱法(GC)、部分酸水解、高碘酸氧化法、Smith 降解法、甲基化反应、红外光谱(IR)、 核磁共振氢谱(1 HNMR) 、刚果红实验和碘-碘化钾实验来分析 PFLP 的结构。经分离纯化后得到两种纯 PFLP 组分(PFLP1、PFLP2) ， 结果分析表明，PFLP1 的分子量范围为9400~9900 u；含有鼠李糖、甘露糖、葡萄糖和半乳糖四种单糖，摩尔比为 1:1.54:18.6:3.64; PFLP2 的分子量范围为 9700~10400 u；含有鼠李糖、木糖、甘露糖、葡萄糖和半乳糖五种单糖，摩尔比为 1:1.08:0.65:6.76:4.28。PFLP1 和 PFLP2 除了都具有 1→2、1→3、1→6 糖苷键，还具有 1→4 糖苷键；都具有典型的多糖红外吸收，是以 β-吡喃糖苷为主并且含有蛋 白质的糖缀合物的多糖；并且都具有三股螺旋结构，有较长的侧链和分支。 关键词：阿魏菇；多糖；单糖组成；结构 文章篇号：1673-9078(2015)3-29-37 DOI: 10.13982/j.mfst.1673-9078.2015.3.006 Structural Analysis of Polysaccharides from Pleurotus ferulae Lenzi CHEN Shuai, XU Cheng-jian, LI Ying-biao, LIU Bing, WANG Xue-ming, HE Yan (College of Food Sciences, Shihezi University, Shihezi 832000, China) Abstract: The Pleurotus ferulae Lenzi polysaccharide (PFLP) was isolated and purified to study its components and structure. The relative molecular mass and distribution of PFLP were analyzed by high-performance gel filtration chromatography. The structure was analyzed by gas chromatography (GC), partial acid hydrolysis, periodate oxidation, Smith degradation, methylation, infrared spectroscopy (IR), 1HNMR, Congo red experiment, and iodine-potassium experiment. Two components, PFLP1 and PFLP2, were obtained through extraction and purification. The result indicated that the relative molecular mass and distribution of PFLP1 was 9400~9900 u. PFLP1 was composed of rhamnose, mannose, glucose, and galactose with a molar ration of 1:1.54:18.6:3.64. The relative molecular mass and distribution of PFLP