响应面法优化超声辅助提取金银木果实色素工艺.pdfVIP

48 2013, Vol.34, No.22 食品科学 ※工艺技术 响应面法优化超声辅助提取金银木果实色素工艺 王立志，唐克华* (吉首大学 林产化工工程湖南省重点实验室，湖南 张家界 427000) 摘  要：对金银木破碎果实首先用溶剂恒温浸提，然后进行超声萃取处理以提取色素。采用响应面优化金银木果实 色素超声辅助提取工艺的料液比、超声时间和超声功率。结果表明：料液比、超声时间和超声功率对金银木果实色 1 15(g mL) 30min 500W 素超声辅助提取均有显著影响；最优工艺条件为料液比 : / 、超声时间 和超声功率 ；在最优工艺 10 450nm 0.836 条件下超声提取的金银木果实色素样液稀释 倍后，在 波长处的吸光度理论预测值为 ，而实际实验的吸 光度为0.827，二者数值仅相差1.08%。因此，运用响应面法优化的超声辅助提取工艺条件可行。 关键词：金银木；色素；超声提取；响应面法 Optimizing Ultrasonic-Assisted Extraction of Pigment from Lonicera maackii Fruit by Response Surface Methodology WANG Li-zhi ，TANG Ke-hua* (Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, China) Abstract ：Following a period of spontaneous solvent extraction at a constant temperature, ultrasonication was applied to enhance pigment extraction from crushed Lonicera maackii fruit. Three extraction parameters including material/solvent ra- tio, ultrasonication time and power were optimized by using response surface methodology. All the operating parameters had a significant effect on extraction efficiency and the optimal extraction conditions were determined as 1:15 (g mL), 30 min / and 500 W for material/solvent ratio, ultrasonication time and power, respectively. The predicted absorbance at 450 nm of the pigment extracted under the optimized conditions after 10-fold dilution was 0.