天麻细胞级微粉碎和普通粉碎质量标准对比实验研究.pdfVIP

天麻细胞级微粉碎和普通粉碎质量标准对比实验研究 刘 智 王 丽(贵阳550001贵州益康制药有限公司) 摘要 目的：比较天麻细胞级微粉碎和普通粉碎在显微鉴别、天麻素及其他成分含量测定等方面的差异。方法：同一批次天 麻分别用细胞级粉碎设备和普通设备粉碎成超微粉和普通粉，用显微镜观察和 HPI C法测定两者的天麻素和其他成分含量。 结果：天麻超微粉不但是天麻素含量比普通粉高得多，而且其他成分也高得多。结论：天麻细胞级微粉碎和普通粉碎在显微 鉴别、天麻素及其他成分含量测定方面均有差异；证明天麻经细胞级微粉碎后的超微粉，可以缩短提取时间，提高活性成分提 取率。 关键词 天麻；细胞级微粉碎；超微粉；天麻素；高效液相色谱法 The experimental studies on quality standard of cellular level and ordinary pulverization for Gastrodia elata BL． Liu Zhi，Wang Li(Guizhou EAKAN Pharmaceutical Co．，Ltd．，Guiyang 550001) ABSTRACT OBJECTIVE：To compare micro identification，content of gastrodine and other ingredients of cellular level pulveri— zation with those of ordinary pulverization for Gastrodia elata BL．．METHOD：The same batch of Gastrodia elata BL．was pul— verized into ultra—fine powder and ordinary powder by cellular level and ordinary pulverizer respectively，the characterizations of powder were indentified by micro identification and content determination of gastrodine and other ingredients were measured by HPLC．RESULTS：In addition tO gastrodine，the content of other ingredients were much higher in ultra—fine powder than those in ordinary powder．CONCLUSION：There was significant difference of micro identification，content of gastrodine and other in— gredients between methods of cellular level and ordinary pulverization；Extracting time of active ingredients can be reduced and Extracting rate can be increased simultaneously by cellular level pulverizing． KEY WORDS Gastrodia elata BL．，cellular level pulverizing，ultra—fine powder，Gastrodine，HPI C 细胞级微粉碎是以植物类药材细胞破壁为目的的粉碎 ～ 180~m)。 作业，并不以细度为目的。天麻超微粉是使用细胞级粉碎设 2．2 天麻超微粉、普通粉显微鉴别对照 备，将天麻药材粉碎制成；粉碎特点是将天麻的细胞壁打破， 取天麻超微粉、普通粉分别制成显微鉴别玻片，置显微 天麻细胞内的活性成分，在溶剂存在的情况下，可以不通过 镜下观察并拍摄照片 细胞壁或膜，直接提取出来，遗留少，从而提高和加快药物提 显微镜下观察天麻普通粉，显示：厚壁细胞为多角形，直 取率。普通粉碎是利用一般粉碎设备将天麻药材粉碎。具 径 7O～180p．m，壁厚约为 3～8 m，木化；含糊化多糖类物的 体实验以