昆虫杆状病毒细胞表达系统 课件.pdf

5.2.2 5.2.2 — — • • • • 1) Functional activity of the recombinant protein The BEVS typically produces overexpressed recombinant proteins containing properfolding, disulfide bond formation and oligomerization. Additionally, this system is capable of performing several post-translational modifications. This leads to a protein that is similar to its native counterpart, both structurally and functionally. However, in cases where the authentic protein functions as a heterodimer or relies on tissue- or species-specific modifications, the recombinant Baculovirus-expressed protein may not be functionally active, unless its binding partner or modifying enzyme is co- expressed. 2) Post-translational modifications 2) Post-translational modifications Several post-translational modifications have been reported to Several post-translational modifications have been reported to occur in the BEVS, including N- and O-linked glycosylation, occur in the BEVS, including N- and O-linked glycosylation, phosphorylation, a