研究与技术鲜茧生丝多酚微量组分提取方法王聪磊1马明波1.DOC

研究与技术 鲜茧生丝多酚微量组分提取方法 王聪磊1，马明波1，潘璐璐2，董锁拽2，唐志荣1，周文龙1 (1.浙江理工大学 材料与纺织学院，杭州 310018；2.浙江出入境检验检疫局 中心，杭州 310012) 摘要为对鲜茧生丝微量组分的提取方法进行探究，文通过高效液相色谱(HPLC)研究了不同质量分数乙醇/水溶液和提取条件(温度、时间和浴比)对鲜茧生丝多酚微量组分提取效率的影响，并采用正交试验优选出较佳提取条件。结果表明，不同溶剂鲜茧生丝提取物的HPLC图有明显差异，表现为乙醇质量分数越高，色谱峰强度越低，组分含量越低。不同提取条件对保留时间为1016 min组分含量均有影响，其中以温度的影响最为显著。研究表明纯水是鲜茧生丝多酚微量组分最佳提取溶剂，以纯水为提取溶剂的较佳提取条件为：温度40 ，时间90 min，浴比1︰25。 关键词生丝；鲜茧；多酚；提取；高效液相色谱 中图分类号：TS102.1 文献标志码：A 文章编号：1001-7003(2017)00-0000-00 引用页码： 收稿日期：2016-11-08修回日期：2017-00-00 基金项目：质检总局科技项目(2016IK282) 作者简介：王聪磊(1990—)，男，硕士研究生，研究方向为新型纺织材料与绿色纺织品。通信作者：周文龙，教授，wzhou@zstu.edu.cn。 xtraction method of polyphenols trace components in fresh raw silk WANG Conglei1, MA Mingbo1, PAN Lulu2, DONG Suozhuai2, TANG Zhirong1, ZHOU Wenlong1 (1.College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, China; 2.Silk Inspection Center, Zhejiang Entry-Exit Inspection Quarantine Bureau, Hangzhou 310012, China) Abstract: To explore the extraction method of trace components in fresh raw silk, this paper is intended to study the effect of extraction solvents (ethanol/water solution) of different mass fractions and extraction conditions (temperature, time and liquor ratio) on extraction efficiency of polyphenols trace components in fresh raw silk via high performance liquid chromatography (HPLC), figure out the optimum extraction conditions via orthogonal test. The results indicate that the HPLC chromatograms of extracts from fresh raw silk with different solvents significantly differ. Specifically, with the mass fraction of ethanol is in negative correlation with both the intensity of chromatographic peaks and the content of components. All the extraction conditions have impact on the content of components having retained for 10 to 16min, and the impact of temperature is most significant. Meanwhile, researches show that pure water is the best extraction solvent of polyphenols trace components in fresh raw silk, of which the optimum extraction conditions a