鹌鹑高尿酸血症动物模型建立及脂代谢紊乱研究-中国试验动物学报.DOC

鹌鹑高尿酸血症动物模型脂代谢研究 马丽1，曾贵荣 1,2，张妙红1，邓青1，张丹1，罗术佳1，潘善庆1，姜德建1，王小青 （1.湖南省药物安全评价研究中心，长沙 410331；2.北京协和医学院药用植物所，北京 100193） []：目的 研究鹌鹑高尿酸血症动物模型及脂代谢。方法 采用高脂饲养鹌鹑30天后，灌胃给予腺嘌呤(50mg/只)，连续7天，建立鹌鹑高尿酸血症模型，观察22天后，取血检测血尿酸、黄嘌呤氧化酶、肌酐(CRE)、尿素氮(BUN)、甘油三脂（TG）、低密度脂蛋白（LDL）、高密度脂蛋白（HDL）、总胆固醇（CHO）、黄嘌呤氧化酶（XOD）、肝脂酶(HL)、脂蛋白脂酶(LPL)、腺苷脱氢酶（ADA)脂质代谢指标，取关节和肾脏进行组织病理学检查。结果 模型对照组动物血尿酸均明显升高（P＜0.01），CRE、BUN、TG、LDL、XOD均有明显升高（P＜0.05或P＜0.01），血清CHO、LPL、ADA、HDL含量明显降低（P＜0.05）。造模后动物足趾关节明显肿大，周围软组织肿胀。X光显示足趾骨密度减低，部分关节面破坏呈溶骨性破坏。病理学检查可见关节滑膜增生，有大量炎症细胞浸润，部分被纤维结缔组织替代，肾脏组织肾小管及间质可见尿酸盐结晶，部分小管轻度扩张，肾间质纤维增生，并伴有大量炎症细胞浸润。结论 该模型具有关节肿大、肾脏尿酸盐沉积等肾脏损伤，且伴有脂代谢紊乱。 [关键词] 鹌鹑；高尿酸血症；脂代谢 Lipid metabolism study quail high uric acid hematic disease animal model MA Li，ZENG Gui-rong,ZHANG Miao-hong,DENG Qing,ZHANG Dan,PAN Shan-qing, JIANG De-jian1, WANG Xiao-qing （1.Hunan Center for Drug Safety Evaluation,Changsha 410331;3.Institute of Medicinal Plant Beijing Union Medical College,Beijing 100193） Abstract: Objective: To uail high uric acid hematic disease animal model and lipid metabolism.Methods:Quail were used high fat feed after thirty days, gastric gavage adenine (50 mg/only)seven days in a row. Quail high uric acid hematic disease animal model was set up.Observed in twenty-two days,blood was tested blood uric acid,xanthine oxidase,creatinine(CRE),urea nitrogen(BUN),triglycerides(TG),low density lipoprotein cholesterol(hdl-c),xanthine high density lipoprotein hdl oxidase(XOD),hepatic lipase(HL),lipoprotein lipase(LPL),adenosine dehydrogenase(ADA)lipid metabolism index,joints and kidneys for histopathological examination.Results: Model control group animals blood uric acid were significantly increased (P0.01), CRE,BUN,TG, LDL and XOD were significantly increased(P0.05or P0.01), serum CHO、LPL、ADA、HDL content significantly decreased (P0.05).Animal toes jointed obvious swollen after building, surrounding soft tissue swelling. X-ray showed toes bone density decrease, part of the joint surface damage is dissolved bony destruction. Patholog