丹参酮ⅡA对肝纤维化大鼠肝组织胶原表达影响.docVIP

丹参酮ⅡA对肝纤维化大鼠肝组织胶原表达影响 　 作者：覃筱燕，严莉，唐丽，戴景峰 【摘要】 目的探讨丹参酮ⅡA(TanshinoneⅡA，TSN)对肝纤维化大鼠肝组织胶原纤维表达的作用。方法采用四氯化碳(CCl4)诱导大鼠肝纤维化动物模型。将Wistar大鼠随机分为空白对照组，CCl4模型组，阳性对照联苯双酯组(100mg/kg)，TSN高、中、低(21.3，14.2，7.1 mg/kg)剂量组，均以灌胃的方式给药。治疗结束后,动物采血处死，分离血清进行血清总蛋白(TP)和白蛋白(ALb)含量检测，以及肝组织匀浆羟脯氨酸(Hyp)的含量检测，观察TSN对慢性肝纤维化大鼠的肝纤维生化指标的影响;取固定部位肝组织做HE染色和Masson胶原纤维特殊染色病理组织学检查，观察肝组织中胶原纤维表达的情况。结果TSN可以恢复肝纤维化大鼠血清中已降低的TP和ALb含量，并下调肝组织Hyp的含量(Plt;0.05或Plt;0.01); HE染色显示TSN可以明显改善肝纤维化的病理损伤;Masson胶原纤维特殊染色发现TSN可以显著降低肝纤维化大鼠肝组织中的胶原纤维的表达。结论TSN对肝组织中胶原纤维的的合成过程具有显著的抑制作用,从而能阻断肝纤维化的病理过程。 【关键词】 丹参酮ⅡA; 肝纤维化; 胶原纤维; Masson 　　Abstract：ObjectiveTo investigate the effect of TanshinoneⅡA(TSN) on the expression of hepatic collagen fibers in rats with liver fibrosis. MethodsHepatic fibrosis was induced by intragastric carbon tetrachloride. The wistar rats were randomly divided into 6 groups：control group, carbon tetrachloride group, bifendate positive control group(100 mg/kg), TSN high, middle, low (21.3,14.2,7.1mg/kg)dose groups. All medicines were given by intragastric administration. Total protein (TP) and albumin (ALb) content in serum were detected in all groups . Hydroxyproline(Hyp) content and histopathology of liver tissue were determined .ResultsTSN could increase the levels of TP, ALb in serum and decreased Hyp level in liver tissue of hepatic fibrosis rats. HE and Masson staining showed that TSN could significantly reduce the expression of collagen fibers of liver tissue in hepatic fibrosis rats. ConclusionTSN can significantly inhibit the synthesis process of collagen fibers in hepatic fibrosis rats, so that it can block the pathological process of liver fibrosis. 　　Key words： TanshinoneⅡA; Hepatic fibrosis; Collagen fibers; Masson 　　已有的研究表明[13],各种原因导致的急、慢性肝损伤进程中由于肝内纤维生成与降解失衡，导致肝脏细胞外基质( ECM)中过多的胶原纤维在肝内沉积而诱发肝纤维化。其病理变化表现为肝内胶原纤维异常增生，肝细胞变性坏死，肝组织结构和功能破坏。丹参为唇形科鼠尾草属植物的干燥根部，为常用中药，有活血祛淤、安神宁心、止痛等功效，其药理及临床应用范围较广。其脂溶性部分有效成分丹参酮ⅡA(TanshinoneⅡA，TSN)已被证明具有抗氧化性、抗急性肝损伤和抗肝纤维化等作用[46]。为了研究TSN对慢性肝纤维化大鼠肝组织胶原表达的作用，本研究采用四氯化碳(CCl4)建立慢性肝纤维化大鼠模型，进一步明确TS