丹参注射液对梗阻性肾病大鼠肾功能及TGFβ1表达影响.docVIP

丹参注射液对梗阻性肾病大鼠肾功能及TGFβ1表达影响 　作者：桂泽红 代睿欣 邓时贵 韩凌 孙静 石灵春 【摘要】 ［目的］观察丹参注射液对梗阻性肾病大鼠肾组织转化生长因子β1（TGFβ1）表达、肾功能及肾病理组织学的影响。［方法］采用半结扎法建立尿路梗阻性肾病大鼠模型，观察丹参注射液治疗性给药对尿路梗阻性肾病大鼠肾组织TGFβ1表达（RTPCR法）、血清肌酐（碱性苦味酸法）、尿素氮（尿素酶法）及肾病理组织学（常规He染色）的影响。［结果］1、大鼠尿路梗阻3d，模型对照组血清肌酐、尿素氮均升高，其中尿素氮升高明显，有显著性差异，但血肌酐升高不明显；丹参治疗组结果类似。术后3d模型对照组和丹参治疗组血清肌酐、尿素氮比较，均Pgt;0.05。大鼠尿路梗阻14d血清肌酐、尿素氮持续升高。丹参治疗组血清肌酐、尿素氮均降低，其中尿素氮降低明显，但仍高于假手术组。大鼠尿路梗阻56d，模型对照组血清尿素氮在高位水平，而血肌酐变化不明显；丹参治疗组结果类似。2、与假手术组比较，模型组肾TGFβ1mRNA呈高表达趋势；丹参治疗组各时点对肾组织TGFβ1mRNA表达未见明显影响，但趋势显示丹参治疗给药可能有轻微抑制TGFβ1mRNA表达。3、病理结果：模型组肾小球及肾小管呈固缩性改变，肾小管广泛坏死；丹参治疗组肾小球及肾小管呈固缩性改变，肾小球个别坏死。［结论］尿路梗阻性肾病大鼠长期注射丹参注射液有助于减少肾小球坏死、改善肾脏功能。 【关键词】 梗阻性肾病；丹参注射液；转化生长因子β1；大鼠 　　Abstract：［Objective］To observe the effect of Salvia Injection on obstructive nephropathy rats kidney function,TGFβ1 expression,pathology and histology,primarily discuss its protective mechanism.［Method］Take halfligation to set rats model of urinary obstruction,observe the effect of Salvia Injection on their TGFβ1 expression(RTPCR method),serum creatinine(picric acid method),BUN(urease method),pathology and histology(routine He Dye).［Result］1.After 3d,in model control group,the serum creatinine and BUN are increased,the latter is more apparent,with marked difference;that of Salvia Injection treatment is similar,all Pgt;0.05.After 14d,they are continually increased;but that in Salvia treatment group is decreased,among which BUN is more apparent,but still higher than false operation group.After 56d,the serum BUN is in high level in model control group,while creatinine changes little;Salvia treatment group has similar result.2.Compared to false operation group,the TGFβ1mRNA expression in model group is in high level;there’s no marked effect of Salvia treatment group on TGFβ1mRNA in every time point,but the trend shows Salvia may slightly inhibit its expression.3.For the pathological result: in model group,the glomerular and tubular are in shrinkage change,the tubular is in broad putrescence;so is in Salvia treatment gro