两种制备方法紫癜方治疗ITP模型小鼠药效探究.docVIP

两种制备方法紫癜方治疗ITP模型小鼠药效探究 　【摘要】 观察两种制备方法的紫癜方对ITP模型小鼠的治疗作用。［方法］采用免疫法建立ITP模型，设立正常组（C）、模型组（M）、醋酸泼尼松组（DC）、血美安组（X）、紫癜水提组（S）和紫癜工艺组（G），利用全自动多物种五分类动物血液分析仪于实验前和实验第14天检测各组小鼠血小板数，实验前、第7天和14天分别记录各组小鼠体重，实验结束记录各组小鼠皮下出血程度。［结果］造模前各组小鼠体重和血小板计数均无显著性差异。实验第7天，M组小鼠体重低于C组，有下降趋势，但无显著性差异；DC组、X组、S组和G组小鼠体重高于M组，有上升趋势，但无显著性差异；其它各组小鼠体重比较，无显著性差异。实验第14天，M组小鼠体重和血小板计数明显低于C组，较C组出现明显瘀斑，DC组、X组、S组和G组小鼠体重均低于C组，血小板计数明显低于C组；DC组、X组、S组和G组小鼠体重和血小板计数均明显高于M组，DC组、S组和G组小鼠皮肤瘀斑较M组明显减轻，X组小鼠皮肤瘀斑与M组比较无显著性差异；X组、S组和G组小鼠血小板计数明显低于DC组；S组和G组小鼠血小板计数明显高于X组；其它各组小鼠体重和血小板计数比较，无显著性差异。［结论］紫癜方的两种制备方法对其治疗ITP的药效作用无影响。 【关键词】 特发性血小板减少性紫癜；紫癜方；制备方法；药效研究 ［Qbjective］To observe the effects of Zidian prescription extracted by two kinds of process on ITP mice. ［Methods］ Using APS to inject into mice to induce the ITP model. Mice were divided into 6 groups: C, M, DC, X, S and G groups. The number of platelet on 7th day and 14th day were measured in mice. Meanwhile the weight on 7th day and 14th day and the degree of subcutaneous bleeding were recorded. ［Results］There is no significant difference in weight and the number of platelet in all groups before the experiment. Compared with the C group, the weight is in down trend in M group on 7th day, the weight and the number of platelet were declined in M group on 14th day, the petechia was more clearly in M group. Compared with the M group, the weight is up trend in DC,X,S and G groups on 7th day, the weight and the number of platelet are enhanced in DC,X,S and G groups on 14th day, the petechia was decreased in DC, S and G groups significantly. Compared with the DC group, the number of platelet is decreased in X,S and G groups. Compared with the X group, the number of platelet is increased in S and G groups. ［Conclusion］ Zidian prescription extracted by two kinds of extraction process has an remedial effect on ITP mouse. There is no significant difference between the two kinds of extraction process. 　　Key words: idiopathic thrombocytopenic purpura; Zidian prescription; extraction process; pharmaco