HCN4基因在风心二狭心房纤颤患者心房组织中mRNA表达浅谈.docVIP

HCN4基因在风心二狭心房纤颤患者心房组织中mRNA表达浅谈 　 作者：周华富，杨涛，冼磊，冯旭，陈铭伍 【摘要】 目的 通过分析风湿性心脏病二尖瓣狭窄患者心房肌组织HCN4基因表达与心房颤动发生的关系，为风心二狭房颤发生机制奠定理论基础。方法 取手术患者心耳组织100 mg，提取RNA后应用半定量逆转录－PCR方法检测mRNA含量，并对PCR产物测序分析。以HCN4基因mRNA和内参β-actin含量的比值作为评价HCN4基因mRNA表达水平指标。结果 测定HCN4基因cDNA 序列同源性为100%。试验组及对照组HCN4基因mRNA与β-actin含量的相对表达值比值（±s）分别为0.696±0.257和0.125±0.010，两组比较，差异有显著性(Plt;0.01）。结论 HCN4基因的过度转录表达可能参与了调控风心二狭房颤的发生过程，揭示了HCN4基因作用的分子机制。 【关键词】 HCN4基因；风心二狭；心房纤颤；序列测定 Increased atrial expression of HCN4 gene mRNA in atrial fibrillation patients with mitral stenosis of rheumatic heart disease ［Abstract］ Objective By analyzing the relationship between the atrial fibrillation patients of mitral stenosis of rheumatic heart disease and HCN4 gene in muscles of right atrium，constructing the theorical basis of development of atrial fibrillation ocurring in mitral stenosis of rheumatic heart disease.Methods Semi-quantitative reverse transcription-PCR(RT-PCR） was used to detect and quantify the mRNAs of HCN4 gene in the atria of two groups with atrial fibrillation(thirty eight patients） or without atrial fibrillation(fourteen patients），then we analysised the sequence of PCR products of HCN4 gene，evaluated of HCN4 gene mRNA with the ratio of starting quantity of HCN4 gene and house-keeping gene β-actin.Results HCN4 genes homology was 100 percent with the BLAST process in genebank of NCBI.The relative expression value （±s） in experimental group and control group was repective 0.696±0.257 and 0.125±0.010，mRNA of HCN4 gene in ataria increased significantly compared with control group (Plt;0.01）.Conclusion The overexpression of HCN4 gene might participate in the progress of atrial fibrillation development in mitral stenosis of rheumatic heart disease，which reveals the molecular mechanism of one function of HCN4 gene. ［Key words］ HCN4 gene；mitral stenosis of rheumatic heart disease；atrial fibrillation；sequence analysis HCN4基因属于超级化激活环核苷酸门控阳离子通道基因家族（HCN），参与编码超极化激活阳离子电流（If）通道结构蛋白，是心脏动作电位的缓慢的舒张期自动去极化激活的一个重要组成部分，参与窦房结细胞自发性舒张期膜去极化