CK20及NMP22在尿路上皮癌中表达及意义.docVIP

CK20及NMP22在尿路上皮癌中表达及意义 　 作者：林毅，陈文轩，强万明，李liming 【摘要】 　　目的 探讨细胞角蛋白20（CK20）和核基质蛋白22（NMP22）在尿路上皮癌中的表达和意义。方法 应用RT-PCR法和酶联免疫法（ELISA）分别检测30例尿路上皮癌（26例移行细胞癌、2例鳞癌、1例腺癌、1例内翻性乳头状癌）、8例正常尿路上皮组织标本中CK20mRNA表达和尿中NMP22的水平。结果 CK20mRNA在30例尿路上皮癌中阳性表达率为93.3%。NMP22在尿路上皮癌中的含量明显高于对照组，分别为（20.7±9.8）u/ml和（6.1±0.8）u/ml，Plt;0.01，而且与肿瘤病理分级和临床分期明显相关。NMP22诊断尿路上皮癌的敏感性、特异性分别为63.5%和65.9%。结论 CK20和NMP22的表达与尿路上皮癌的生物学行为密切相关。NMP22与尿路上皮癌的恶性程度呈正相关，CK20和NMP22可协同参与尿路上皮癌的生物学行为。 【关键词】 尿路上皮癌 细胞角蛋白20 核基质蛋白22 　　Expression and significance of cytokeratin 20 mRNA and nuclear matrix protein 22 in urothelial cancer ［Abstract］ Objective To study the correlation of cytokeratin 20 （CK20） mRNA and nuclear matrix protein 22 （NMP22） in urothelial cancer.Methods RT-PCR was used to determine the expression of CK20 mRNA in 30 urothelial cancers （26 transitional cell carcinomas，2 phosphrote carcinomas，1 adeno carcinoma，1 inverted papilloma），and 8 normal tissues.NMP22 levels in urinary were determined by an enzyme-linked immunosorbent assay （ELISA） in urothelial cancers.Results The positive rate of CK20 mRNA in 30 urothelial cancer was 93.3%.The median NMP22 value was significantly higher than control group ［（20.7±9.8）u/ml versus （6.1±0.8）u/ml］ in urothelial cancer.The values of NMP 22 was found to be positively correlated to the degree of the differentiation and the clinical staging of the urothelial cancer.The sensitivity and specificity of NMP22 were 63.5% and 65.9%，respectively.Conclusion The results suggest that CK20 mRNA and NMP 22 play important role in the occurrence and development of urothelial cancer.These findings suggest that the detection of multiple factors is more valuable than that of single factor. ［Key words］ urothelial tumor；cytokeratin20；nuclear matrix protein 22 尿路上皮癌是泌尿系统最常见的恶性肿瘤，其中90%以上为移行细胞癌，其特点为术后复发率高，往往复发后其恶性度增大，而且肿瘤的浸润和转移倾向增强，肿瘤的生物学行为受多种基因的调控。近年发现CK20只在胃肠道和尿路上皮中表达，核基质蛋白（NMP）对DNA的复制、转录以及基因表达的调节起着重要作用。我们采用RT-PCR法检测细胞角蛋白20（CK20）mRNA的表达，应用酶联免疫法（ELISA）测定尿路上皮癌中核基质蛋白22（NMP22）的水平，探讨其与尿路上皮癌的生物学行为的关系，现报告如下。