detection and characterizationof cellular immune responses using peptide–mhc microarrays使用peptide-mhc微阵列检测和characterizationof细胞免疫反应.pdfVIP

PLoS BIOLOGY Detection and Characterization of Cellular Immune Responses Using Peptide–MHC Microarrays 1[ 2,5[ 3 4,5* 1,5* Yoav Soen , Daniel S. Chen , Daniel L. Kraft , Mark M. Davis , Patrick O. Brown 1 Department of Biochemistry, Stanford University, Stanford, California, United States of America, 2 Department of Internal Medicine, Division of Oncology, Stanford University, Stanford, California, United States of America, 3 Department of Pediatrics, Division of Bone Marrow Transplant, Stanford University, Stanford, California, United States of America, 4 Department of Microbiology and Immunology, Stanford University, Stanford, California, United States of America, 5 Howard Hughes Medical Institute, Stanford University, Stanford, California, United States of America The detection and characterization of antigen-specific T cell populations is critical for understanding the development and physiology of the immune system and its responses in health and disease. We have developed and tested a method that uses arrays of peptide–MHC complexes for the rapid identification, isolation, activation, and characterization of multiple antigen-specific populations of T cells. CD4þ or CD8þ lymphocytes can be captured in accordance with their ligand specificity using an array of peptide–MHC complexes printed on a film-coated glass surface. We have characterized the specificity and sensitivity of a peptide–MHC array using labeled lymphocytes from T cell receptor transgenic mice. In addition, we were able to use the array to detect a rare population of antigen-specific T cell