detection and localisation of prpsc in the liver of sheep infected with scrapie and bovine spongiform encephalopathy检测和本地化的朊蛋白在肝脏羊痒病感染和牛海绵状脑病.pdfVIP

Detection and Localisation of PrPSc in the Liver of Sheep Infected with Scrapie and Bovine Spongiform Encephalopathy 1 1 1 1 1 Sally J. Everest , Andrew M. Ramsay , Melanie J. Chaplin , Sharon Everitt , Michael J. Stack , Michael H. 1 2 1 1 1 Neale *, Martin Jeffrey , S. Jo Moore , Susan J. Bellworthy , Linda A. Terry 1 Department of Pathology and Host Susceptibility, Veterinary Laboratories Agency, New Haw, United Kingdom, 2 Department of Pathology and Host Susceptibility, Veterinary Laboratories Agency (VLA-Lasswade), Penicuik, Midlothian, United Kingdom Abstract Prions are largely contained within the nervous and lymphoid tissue of transmissible spongiform encephalopathy (TSE) infected animals. However, following advances in diagnostic sensitivity, PrPSc, a marker for prion disease, can now be located in a wide range of viscera and body fluids including muscle, saliva, blood, urine and milk, raising concerns that exposure to these materials could contribute to the spread of disease in humans and animals. Previously we demonstrated low levels of infectivity in the liver of sheep experimentally challenged with bovine spongiform encephalopathy. In this study we show that PrPSc accumulated in the liver of 89% of sheep naturally infected with scrapie and 100% of sheep challenged with BSE, at both clinical and preclinical stages of the disease. PrPSc was demonstrated in the absence of obvious inflammatory foci and was restricted to isolated resident cells, most likely Kupffer cells. Citation: Everest SJ, Ramsay AM, Chaplin MJ, Everitt S, Stack MJ, et al. (2011) Det