de-novo assembly and analysis of the heterozygous triploid genome of the wine spoilage yeast dekkera bruxellensis awri1499新创的装配和分析杂合的三倍体基因组的葡萄酒败坏酵母dekkera bruxellensis awri1499.pdfVIP

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de-novo assembly and analysis of the heterozygous triploid genome of the wine spoilage yeast dekkera bruxellensis awri1499新创的装配和分析杂合的三倍体基因组的葡萄酒败坏酵母dekkera bruxellensis awri1499.pdf

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de-novo assembly and analysis of the heterozygous triploid genome of the wine spoilage yeast dekkera bruxellensis awri1499新创的装配和分析杂合的三倍体基因组的葡萄酒败坏酵母dekkera bruxellensis awri1499

De-Novo Assembly and Analysis of the Heterozygous Triploid Genome of the Wine Spoilage Yeast Dekkera bruxellensis AWRI1499 1 . 1. 1 1,2 Chris D. Curtin * , Anthony R. Borneman , Paul J. Chambers , Isak S. Pretorius 1The Australian Wine Research Institute, Adelaide, Australia, 2 The University of South Australia, Adelaide, Australia Abstract Despite its industrial importance, the yeast species Dekkera (Brettanomyces) bruxellensis has remained poorly understood at the genetic level. In this study we describe whole genome sequencing and analysis for a prevalent wine spoilage strain, AWRI1499. The 12.7 Mb assembly, consisting of 324 contigs in 99 scaffolds (super-contigs) at 26-fold coverage, exhibits a relatively high density of single nucleotide polymorphisms (SNPs). Haplotype sampling for 1.2% of open reading frames suggested that the D. bruxellensis AWRI1499 genome is comprised of a moderately heterozygous diploid genome, in combination with a divergent haploid genome. Gene content analysis revealed enrichment in membrane proteins, particularly transporters, along with oxidoreductase enzymes. Availability of this assembly and annotation provides a resource for further investigation of genomic organization in this species, and functional characterization of genes that may confer important phenotypic traits. Citation: Curtin CD, Borneman AR, Chambers PJ, Pretorius IS (2012) De-Novo Assembly and Analysis of the Heterozygous Triploid Genome of the Wine Spoilage Yeast Dekkera bruxellensis AWRI1499. PLoS ONE 7(3): e33840. doi:10.1371/journal.pone.0033840 Editor: Cecile Fairhead, Institut de Genetique et Microbiologie, France Received November 8, 2011; Accepted Februa