denaturing gradient gel electrophoresis (dgge) as a powerful novel alternative for differentiation of epizootic isa virus variants变性梯度凝胶电泳(dgge)作为一个强大的新型替代分化的家畜流行病isa病毒变体.pdfVIP
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denaturing gradient gel electrophoresis (dgge) as a powerful novel alternative for differentiation of epizootic isa virus variants变性梯度凝胶电泳(dgge)作为一个强大的新型替代分化的家畜流行病isa病毒变体
Denaturing Gradient Gel Electrophoresis (DGGE) as a Powerful Novel Alternative for Differentiation of Epizootic ISA Virus Variants 1,2 ´ 1,2 ´ 3 1,2 1,2,3 Marisela Carmona , Dagoberto Sepulveda , Constanza Cardenas , Luis Nilo , Sergio H. Marshall * ´ ´ ´ ´ ´ ´ 1 Laboratorio de Patologenos Acuıcolas, Nucleo Biotecnologıa Curauma, Pontificia Universidad Catolica de Valparaıso, Campus Curauma, Valparaıso, Chile, 2 Laboratorio ´ ´ ´ ´ ´ ´ de Genetica e Inmunologıa Molecular, Instituto de Biologıa, Facultad de Ciencias, Pontificia Universidad Catolica de Valparaıso, Campus Curauma, Valparaıso, Chile, ´ ´ ´ ´ ´ 3 Nucleo Biotecnologıa Curauma, Pontificia Universidad Catolica de Valparaıso, Valparaıso, Chile Abstract Infectious Salmon Anemia is a devastating disease critically affecting world-wide salmon production. Chile has been particularly stricken by this disease which in all cases has been directly related with its causative agent, a novel orthomyxovirus which presents specific and distinctive infective features. Among these, two molecular markers have been directly associated with pathogenicity in two of the eight RNA sub genomic coding units of the virus: an insertion hot spot region present in viral segment 5 and a Highly Polymorphic Region (HPR) located
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