有哪些信誉好的足球投注网站defining the sister rat mammary tumor cell lines hh-16 cl.21 and hh-16.cl.4 as an in vitro cell model for erbb2定义妹妹大鼠乳腺肿瘤细胞株hh-16 cl。.pdfVIP
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defining the sister rat mammary tumor cell lines hh-16 cl.21 and hh-16.cl.4 as an in vitro cell model for erbb2定义妹妹大鼠乳腺肿瘤细胞株hh-16 cl。
Defining the Sister Rat Mammary Tumor Cell Lines HH-16 cl.2/1 and HH-16.cl.4 as an In Vitro Cell Model for Erbb2 1 1 1,2 Sandra Louzada , Filomena Adega , Raquel Chaves * ´ 1 Center of Genomics and Biotechnology, Institute for Biotechnology and Bioengineering, University of Tras-os-Montes and Alto Douro (IBB/CGB-UTAD), Vila Real, ´ Portugal, 2 Department of Genetics and Biotechnology, University of Tras-os-Montes and Alto Douro, Vila Real, Portugal Abstract Cancer cell lines have been shown to be reliable tools in genetic studies of breast cancer, and the characterization of these lines indicates that they are good models for studying the biological mechanisms underlying this disease. Here, we describe the molecular cytogenetic/genetic characterization of two sister rat mammary tumor cell lines, HH-16 cl.2/1 and HH-16.cl.4, for the first time. Molecular cytogenetic analysis using rat and mouse chromosome paint probes and BAC/PAC clones allowed the characterization of clonal chromosome rearrangements; moreover, this strategy assisted in revealing detected breakpoint regions and complex chromosome rearrangements. This comprehensive cytogenetic analysis revealed an increase in the number of copies of the Mycn and Erbb2 genes in the investigated cell lines. To analyze its possible correlation with expression changes, relative RNA expression was assessed by real-time reverse transcription quantitative
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