有哪些信誉好的足球投注网站defensome against toxic diatom aldehydes in the sea urchin paracentrotus lividusdefensome对有毒硅藻醛的海胆paracentrotus lividus.pdfVIP
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defensome against toxic diatom aldehydes in the sea urchin paracentrotus lividusdefensome对有毒硅藻醛的海胆paracentrotus lividus
Defensome against Toxic Diatom Aldehydes in the Sea Urchin Paracentrotus lividus 1 2. 3. 3 1 Vincenzo Marrone , Marina Piscopo , Giovanna Romano , Adrianna Ianora , Anna Palumbo , Maria Costantini1* 1 Laboratory of Cellular and Developmental Biology, Stazione Zoologica Anton Dohrn, Naples, Italy, 2 Department of Structural and Functional Biology, University of Naples Federico II, Naples, Italy, 3 Laboratory of Functional and Evolutionary Ecology, Stazione Zoologica Anton Dohrn, Naples, Italy Abstract Many diatom species produce polyunsaturated aldehydes, such as decadienal, which compromise embryonic and larval development in benthic organisms. Here newly fertilized Paracentrotus lividus sea urchins were exposed to low concentration of decadienal and the expression levels of sixteen genes, implicated in a broad range of functional responses, were followed by Real Time qPCR in order to identify potential decadienal targets. We show that at low decadienal concentrations the sea urchin Paracentrotus lividus places in motion different classes of genes to defend itself against this toxic aldehyde, activating hsp60 and two proteases, hat and BP10, at the blastula stage and hsp56 and several other genes ( 14-3-3e, p38 MAPK, MTase, and GS) at the prism stage. At this latter stage all genes involved in skeletogenesis (Nec, uni, SM50 and SM30) were also down-expressed, following developmental abnormalities that mainly affected skeleton morphogenesis. Moreover, sea urchin embryos treated with increasing concentrations of decadienal revealed a dose- dependent response of
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