de novo biosynthesis of biodiesel by escherichia coli in optimized fed-batch cultivation新创生物合成生物柴油的大肠杆菌在优化馈料式种植.pdfVIP

De novo Biosynthesis of Biodiesel by Escherichia coli in Optimized Fed-Batch Cultivation Yangkai Duan., Zhi Zhu., Ke Cai, Xiaoming Tan, Xuefeng Lu* Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China Abstract Biodiesel is a renewable alternative to petroleum diesel fuel that can contribute to carbon dioxide emission reduction and energy supply. Biodiesel is composed of fatty acid alkyl esters, including fatty acid methyl esters (FAMEs) and fatty acid ethyl esters (FAEEs), and is currently produced through the transesterification reaction of methanol (or ethanol) and triacylglycerols (TAGs). TAGs are mainly obtained from oilseed plants and microalgae. A sustainable supply of TAGs is a major bottleneck for current biodiesel production. Here we report the de novo biosynthesis of FAEEs from glucose, which can be derived from lignocellulosic biomass, in genetically engineered Escherichia coli by introduction of the ethanol- producing pathway from Zymomonas mobilis, genetic manipulation to increase the pool of fatty acyl-CoA, and heterologous expression of acyl-coenzyme A: diacylglycerol acyltransferase from Acinetobacter baylyi. An optimized fed-batch microbial fermentation of the modified E. coli strain yielded a titer of 922 mg L21 FAEEs that consisted primarily of ethyl palmitate, -oleate, -myristate and -palmitoleate. Citation: Duan Y, Zhu Z, Cai K, Tan X, Lu X (2011) De novo Biosynthesis of Biodiesel by Escherichia coli in Optimized Fed-Batch Cultivation. PLoS ONE 6(5): e20265. doi:10.1371/journal.pone.0020265 Editor: Arnold Driessen, University of Groningen, Netherlands Received December 25, 2010; Accepted April 28, 2011; Published May 23, 2011 Copyright: 2011 Duan et al. This is an open-access article distribu