cystic fibrosis-niche adaptation of pseudomonas aeruginosa reduces virulence in multiple infection hosts囊性fibrosis-niche改编的铜绿假单胞菌感染减少毒性在多个主机.pdfVIP

Cystic Fibrosis-Niche Adaptation of Pseudomonas aeruginosa Reduces Virulence in Multiple Infection Hosts ` 1. 1. 1 1 2 2 Nicola Ivan Lore , Cristina Cigana , Ida De Fino , Camilla Riva , Mario Juhas , Stephan Schwager , 2 1 Leo Eberl , Alessandra Bragonzi * 1 Infection and Cystic Fibrosis Unit, Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy, 2 Department of Microbiology, Institute of Plant Biology, University of Zurich, Zurich, Switzerland Abstract The opportunistic pathogen Pseudomonas aeruginosa is able to thrive in diverse ecological niches and to cause serious human infection. P. aeruginosa environmental strains are producing various virulence factors that are required for establishing acute infections in several host organisms; however, the P. aeruginosa phenotypic variants favour long-term persistence in the cystic fibrosis (CF) airways. Whether P. aeruginosa strains, which have adapted to the CF-niche, have lost their competitive fitness in the other environment remains to be investigated. In this paper, three P. aeruginosa clonal lineages, including early strains isolated at the onset of infection, and late strains, isolated after several years of chronic lung infection from patients with CF, were analysed in multi-host model systems of acute infection. P. aeruginosa early isolates caused lethality in the three non-mammalian hosts, namely Caenorhabditis elegans, Galleria mellonella, and Drosophila melanogaster, while late adapted clonal isolates were attenuated