cyclophilin a restricts influenza a virus replication through degradation of the m1 protein还有限制甲型流感病毒复制通过m1蛋白的降解.pdfVIP

Cyclophilin A Restricts Influenza A Virus Replication through Degradation of the M1 Protein 1. 1,2. 1,2 1 1,2 4 Xiaoling Liu , Zhendong Zhao , Chongfeng Xu , Lei Sun , Jilong Chen , Lianfeng Zhang , Wenjun Liu1,2,3* 1 Center for Molecular Virology, CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China, 2 Graduate University of Chinese Academy of Sciences, Beijing, China, 3 China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China, 4 Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medical Center, Peking Union Medical College, Beijing, China Abstract Cyclophilin A (CypA) is a typical member of the cyclophilin family of peptidyl-prolyl isomerases and is involved in the replication of several viruses. Previous studies indicate that CypA interacts with influenza virus M1 protein and impairs the early stage of the viral replication. To further understand the molecular mechanism by which CypA impairs influenza virus replication, a 293T cell line depleted for endogenous CypA was established. The results indicated that CypA inhibited the initiation of virus replication. In addition, the infectivity of influenza virus increased in the absence of CypA. Further studies indicated that CypA had no effect on the stages of virus genome replication or transcription and also did not impair the nuclear export of the viral mRNA. However, CypA decreased the viral protein level. Additional studies indicated that CypA enhanced th