cyclin d1 expression and the inhibitory effect of celecoxib on ovarian tumor growth in vivo细胞周期蛋白d1表达式和塞来昔布在卵巢肿瘤生长的抑制作用在体内.pdfVIP
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cyclin d1 expression and the inhibitory effect of celecoxib on ovarian tumor growth in vivo细胞周期蛋白d1表达式和塞来昔布在卵巢肿瘤生长的抑制作用在体内
Int. J. Mol. Sci. 2010, 11, 3999-4013; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Cyclin D1 Expression and the Inhibitory Effect of Celecoxib on Ovarian Tumor Growth in Vivo Wei Li *, Hong-Ru Jiang, Xiao-Li Xu, Jie Wang, Jun Zhang, Mei-Lin Liu and Ling-Yun Zhai Department of Gynecology, Nanjing Medical University of Hangzhou Hospital, 261 Huansha Road, Hangzhou, Zhejiang, 310006, China; E-Mails: hongrujiang@ (H.-R.J.); joyce.xiaolixu@ (X .-L.X.); hongsheqiao@ (J.W.); viola0116@163.com (J.Z.); lm163.com (M.-L.L.); 494610422@ (L.-Y.Z.) * Author to whom correspondence should be addressed; E-Mail: wei5901482@; Tel.: +86-0571 Fax: +86-0571 Received: 07 September 2010; in revised form: 28 September 2010 / Accepted: 08 October 2010 / Published: 19 October 2010 Abstract: The report aims to investigate the relationship between the expression of cyclin D1 and Cyclooxgenase-2 (COX-2), thus to explore the molecular mechanisms of the antitumor efficacy of Celecoxib, a COX-2 inhibitor. Human ovarian SKOV-3 carcinoma cell xenograft-bearing mice were treated with Celecoxib by infusing gaster (i.g.) twice/day for 21 days. The mRNA levels of COX-2 and cyclin D1 were determined by RT-PCR. The expression of cyclin D1 at the protein level was detected by immunohistochemistry, while COX-2 protein expression was determined by Western blot. A high-dose
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