ctbp a successful intron length polymorphism (ilp)-based genotyping method targeted to well defined experimental needsctbp成功的基因内区长度多态性(独立)的基因分型方法针对定义良好的实验需求.pdfVIP

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ctbp a successful intron length polymorphism (ilp)-based genotyping method targeted to well defined experimental needsctbp成功的基因内区长度多态性(独立)的基因分型方法针对定义良好的实验需求.pdf

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ctbp a successful intron length polymorphism (ilp)-based genotyping method targeted to well defined experimental needsctbp成功的基因内区长度多态性(独立)的基因分型方法针对定义良好的实验需求

Diversity 2010, 2, 572-585; doi:10.3390/d2040572 OPEN ACCESS diversity ISSN 1424-2818 /journal/diversity Article cTBP: A Successful Intron Length Polymorphism (ILP)-Based Genotyping Method Targeted to Well Defined Experimental Needs Luca Braglia, Antonella Manca, Francesco Mastromauro and Diego Breviario * Institute of Agricultural Biology and Biotechnology (IBBA) -C.N.R Via Bassini 15, 20133 Milan, Italy; E-Mails: braglia@r.it (L.B.); manca@r.it (A.M.); mastromauro@r.it (F.M.) * Author to whom correspondence should be addressed; E-Mail: terenzio@r.it; Tel: +39-02-2369-9441; Fax: +39-02-2369-9411. Received: 20 February 2010; in revised form: 2 April 2010 / Accepted: 8 April 2010 / Published: 15 April 2010 Abstract: There seem to be a certain degree of reluctance in accepting ILP-based methods as part of the range of molecular markers that are classically used for plant genotyping. Indeed, since DNA polymorphism results from difference in length of fragments amplified from specific gene loci, not anonymous sequences, the number of markers that can be generated is sometime inadequate for classical phylogeny studies. Yet, ILP-based markers have many other useful advantages that should not go neglected. We support this statement by presenting a large variety of data we have been collecting for a long while regarding the use of cTBP, an ILP marker based on difference in length of the introns present within the members of the plant beta-tubulin gene family. Keywords: plant