crystal structure of an integron gene cassette-associated protein from vibrio cholerae identifies a cationic drug-binding module整合子基因cassette-associated蛋白的晶体结构霍乱弧菌标识一个阳离子drug-binding模块.pdfVIP

Crystal Structure of an Integron Gene Cassette- Associated Protein from Vibrio cholerae Identifies a Cationic Drug-Binding Module 1 2,3 4¤ 1 5 Chandrika N. Deshpande , Stephen J. Harrop , Yan Boucher , Karl A. Hassan , Rosa Di Leo , Xiaohui Xu5, Hong Cui5, Alexei Savchenko5, Changsoo Chang5, Maurizio Labbate6, Ian T. Paulsen 1, H. W. Stokes6, Paul M. G. Curmi2,3, Bridget C. Mabbutt 1* 1 Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, New South Wales, Australia, 2 School of Physics, University of New South Wales, Sydney, New South Wales, Australia, 3 St Vincent’s Centre for Applied Medical Research, Sydney, New South Wales, Australia, 4 Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America, 5 Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada, 6 Institute for the Biotechnology of Infectious Diseases, University of Technology, Sydney, New South Wales, Australia Abstract Background: The direct isolation of integron gene cassettes from cultivated and environmental microbial sources allows an assessment of the impact of the integron/gene cassette system on the emergence of new phenotypes, such as drug resistance or virulence. A structural approach is being exploited to investigate the modularity and function of novel integron gene cassettes. ˚ Methodology/Principal Findings: We report the 1.8 A crystal structure of Cass2