coordinated progression through two subtranscriptomes underlies the tachyzoite cycle of toxoplasma gondii通过两个subtranscriptomes协调发展基础的tachyzoite周期刚地弓形虫.pdfVIP

Coordinated Progression through Two Subtranscriptomes Underlies the Tachyzoite Cycle of Toxoplasma gondii 1,4. 3. 1 1,2 2 Michael S. Behnke , John C. Wootton , Margaret M. Lehmann , Josh B. Radke , Olivier Lucas , Julie 4 4 1,2 Nawas , L. David Sibley , Michael W. White * 1 Department of Veterinary Molecular Biology, Montana State University, Bozeman, Montana, United States of America, 2 Departments of Molecular Medicine and Global Health, University of South Florida, Tampa, Florida, United States of America, 3 Computational Biology Branch, National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland, United States of America, 4 Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, United States of America Abstract Background: Apicomplexan parasites replicate by varied and unusual processes where the typically eukaryotic expansion of cellular components and chromosome cycle are coordinated with the biosynthesis of parasite-specific structures essential for transmission. Methodology/Principal Findings: Here we describe the global cell cycle transcriptome of the tachyzoite stage of Toxoplasma gondii. In dividing tachyzoites, more than a third of the mRNAs exhibit significant cyclical profiles whose timing correlates with biosynthetic events that unfold during daughter parasite formation. These 2,833 mRNAs have a bimodal organization with peak expression occurring in one of two transcriptional waves that are bounded by the transition into S phase an