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continuous membrane-based screening system for biocatalysis连续为生物催化膜基筛选系统.pdf

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continuous membrane-based screening system for biocatalysis连续为生物催化膜基筛选系统

Membranes 2011, 1, 70-79; doi:10.3390/membranes1010070 OPEN ACCESS membranes ISSN 2077-0375 /journal/membranes/ Article Continuous Membrane-Based Screening System for Biocatalysis Evgenij Lyagin 1,*, Anja Drews 2, Subhamoy Bhattacharya 1,3 and Matthias Kraume 1 1 Chair of Chemical and Process Engineering, Technische Universität Berlin, Sekr. MA 5-7, Straße des 17. Juni 135, 10623 Berlin, Germany; E-Mails: subhamoy@mailbox.tu-berlin.de (S.B.); matthias.kraume@tu-berlin.de (M.K.) 2 HTW Berlin, Department of Engineering II, School of Life Science Engineering, Wilhelminenhofstr. 75a, 12459 Berlin, Germany; E-Mail: anja.drews@htw-berlin.de 3 Institute for Chemistry, Department of Enzyme Technology, Technische Universität Berlin, Sekr. TC 4, Straße des 17. Juni 124, 10623 Berlin, Germany * Author to whom correspondence should be addressed; E-Mail: evgenij.lyagin@tu-berlin.de; Tel.: +49-30-314-726-93; Fax: +49-30-314-727-56. Received: 30 December 2010; in revised form: 17 February 2011 / Accepted: 17 February 2011 / Published: 25 February 2011 Abstract: The use of membrane reactors for enzymatic and co-factor regenerating reactions offers versatile advantages such as higher conversion rates and space-time-yields and is therefore often applied in industry. However, currently available screening and kinetics characterization systems are based on batch and fed-batch operated reactors and were developed for whole cell biotransformations rather than for enzymatic catalysis.