consumption of bt maize pollen expressing cry1ab or cry3bb1 does not harm adult green lacewings, chrysoperla carnea (neuroptera chrysopidae)消费bt玉米花粉表达cry1ab或cry3bb1不会伤害成人绿草蜻蛉,chrysoperla carnea(脉翅目chrysopidae).pdfVIP

Consumption of Bt Maize Pollen Expressing Cry1Ab or Cry3Bb1 Does Not Harm Adult Green Lacewings, Chrysoperla carnea (Neuroptera: Chrysopidae) ¨ Yunhe Li, Michael Meissle, Jorg Romeis* ¨ Agroscope Reckenholz-Tanikon Research Station ART, Zurich, Switzerland Abstract Adults of the common green lacewing, Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae), are prevalent pollen- consumers in maize fields. They are therefore exposed to insecticidal proteins expressed in the pollen of insect-resistant, genetically engineered maize varieties expressing Cry proteins derived from Bacillus thuringiensis (Bt). Laboratory experiments were conducted to evaluate the impact of Cry3Bb1 or Cry1Ab-expressing transgenic maize (MON 88017, Event Bt176) pollen on fitness parameters of adult C. carnea. Adults were fed pollen from Bt maize varieties or their corresponding near isolines together with sucrose solution for 28 days. Survival, pre-oviposition period, fecundity, fertility and dry weight were not different between Bt or non-Bt maize pollen treatments. In order to ensure that adults of C. carnea are not sensitive to the tested toxins independent from the plant background and to add certainty to the hazard assessment, adult C. carnea were fed with artificial diet containing purified Cry3Bb1 or Cry1Ab at about a 10 times higher concentration than in maize pollen. Artificial diet containing Galanthus nivalis agglutinin (GNA) was included as a positive control. No differences were found in any life-table parameter between Cry protein containing diet treatments and control diet. However, the pre- oviposition period, daily and total fecundity and dry weight of C. carnea were significantly negatively affected by GNA- feeding. In both feed