construction of cardiac tissue rings using a magnetic tissue fabrication technique心脏组织建设环使用磁组织制造技术.pdfVIP
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construction of cardiac tissue rings using a magnetic tissue fabrication technique心脏组织建设环使用磁组织制造技术
Int. J. Mol. Sci. 2010, 11, 2910-2920; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Construction of Cardiac Tissue Rings Using a Magnetic Tissue Fabrication Technique Hirokazu Akiyama, Akira Ito, Masanori Sato, Yoshinori Kawabe and Masamichi Kamihira * Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan; E-Mails: hakiyama@chem-eng.kyushu-u.ac.jp (H.A.); akira@chem-eng.kyushu-u.ac.jp (A.I.); 2TE10138G@s.kyushu-u.ac.jp (M.S.); kawabe@chem-eng.kyushu-u.ac.jp (Y.K.) * Author to whom correspondence should be addressed; E-Mail: kamihira@chem-eng.kyushu-u.ac.jp; Tel.: +81-92-802-2743; Fax: +81-92-802-2793. Received: 22 April 2010; in revised form: 26 July 2010 / Accepted: 2 August 2010 / Published: 10 August 2010 Abstract: Here we applied a magnetic force-based tissue engineering technique to cardiac tissue fabrication. A mixture of extracellular matrix precursor and cardiomyocytes labeled with magnetic nanoparticles was added into a well containing a central polycarbonate cylinder. With the use of a magnet, the cells were attracted to the bottom of the well and allowed to form a cell layer. During cultivation, the cell layer shrank towards the cylinder, leading to the formation of a ring-shaped tissue that possessed a multilayered cell structure and contractile properties. These re
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