有哪些信誉好的足球投注网站comprehensive analysis of leukocytes, vascularization and matrix metalloproteinases in human menstrual xenograft model综合分析的白细胞,血管化和基质金属蛋白酶在人类月经异种移植模型.pdfVIP
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comprehensive analysis of leukocytes, vascularization and matrix metalloproteinases in human menstrual xenograft model综合分析的白细胞,血管化和基质金属蛋白酶在人类月经异种移植模型
Comprehensive Analysis of Leukocytes, Vascularization and Matrix Metalloproteinases in Human Menstrual Xenograft Model 1,2 2 2 2 Yong Guo , Bin He *, Xiangbo Xu , Jiedong Wang * 1 Graduate School, Peking Union Medical College, Beijing, People’s Republic of China, 2 Department of Cell Biology, National Research Institute for Family Planning, Beijing, People’s Republic of China Abstract In our previous study, menstrual-like changes in mouse were provoked through the pharmacologic withdrawal of progesterone with mifepristone following induction of decidualization. However, mouse is not a natural menstruation animal, and the menstruation model using external stimuli may not truly reflect the occurrence and development of the human menstrual process. Therefore, we established a model of menstruation based on human endometrial xenotransplantation. In this model, human endometrial tissues were transplanted subcutaneously into SCID mice that were ovarectomized and supplemented with estrogen and progestogen by silastic implants with a scheme imitating the endocrinological milieu of human menstrual cycle. Morphology, hormone levels, and expression of vimentin and cytokeratin markers were evaluated to confirm the menstrual-like changes in this model. With 28 days of hormone treatment, transplanted human endometrium survived and underwent proliferation, differentiation and disintegration, similar to human endometrium in vivo. Human CD45+ cells showed a peak of increase 28 days post-transplantation. Three days after progesterone withdrawal, mouse CD45+ cells increased rapidly in number and were
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