composition and similarity of bovine rumen microbiota across individual animals成分和相似的牛瘤胃微生物群在动物个体.pdfVIP

Composition and Similarity of Bovine Rumen Microbiota across Individual Animals Elie Jami1,2, Itzhak Mizrahi 1* 1 Department of Ruminant Science, Institute of Animal Sciences, Agricultural Research Organization, Bet Dagan, Israel, 2 Department of Molecular Microbiology and Biotechnology, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat-Aviv, Israel Abstract The bovine rumen houses a complex microbiota which is responsible for cattle’s remarkable ability to convert indigestible plant mass into food products. Despite this ecosystem’s enormous significance for humans, the composition and similarity of bacterial communities across different animals and the possible presence of some bacterial taxa in all animals’ rumens have yet to be determined. We characterized the rumen bacterial populations of 16 individual lactating cows using tag amplicon pyrosequencing. Our data showed 51% similarity in bacterial taxa across samples when abundance and occurrence were analyzed using the Bray-Curtis metric. By adding taxon phylogeny to the analysis using a weighted UniFrac metric, the similarity increased to 82%. We also counted 32 genera that are shared by all samples, exhibiting high variability in abundance across samples. Taken together, our results suggest a core microbiome in the bovine rumen. Furthermore, although the bacterial taxa may vary considerably between cow rumens, they appear to be phylogenetically related. This suggests that the functional requirement imposed by the rumen ecological niche selects taxa that potentially share similar genetic features. Citation: Jami E, Mizrahi I (2012) Composition and Similarity of Bovine Rumen Microbiota across Individual Animals. PLoS ONE 7(3): e33306. doi:10.1371/ jour