comparison of quantitative techniques including xpert mtbrif to evaluate mycobacterial burden比较的量化技术,包括爱视宝mtbrif评估分枝杆菌的负担.pdfVIP

Comparison of Quantitative Techniques including Xpert MTB/RIF to Evaluate Mycobacterial Burden 1 1 1 2 1 Richard N. van Zyl-Smit , Anke Binder , Richard Meldau , Hridesh Mishra , Patricia L. Semple , Grant 1 1 3 2 4 1 Theron , Jonathan Peter , Andrew Whitelaw , Suren K. Sharma , Robin Warren , Eric D. Bateman , Keertan Dheda1,5,6* 1 Lung Infection and Immunity Unit, Division of Pulmonology and UCT Lung Institute, Department of Medicine, University of Cape Town, Cape Town, South Africa, 2 Department of Medicine, All India Institute of Medical Sciences, New Delhi, India, 3 Division of Medical Microbiology, University of Cape Town, Cape Town, South Africa, 4 DST/NRF Centre of Excellence for Biomedical TB Research/MRC Centre for Molecular and Cellular Biology, Stellenbosch University, Stellenbosch, South Africa, 5 Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town, South Africa, 6 Department of Infection, University College London Medical School, London, United Kingdom Abstract Introduction: Accurate quantification of mycobacterial load is important for the evaluation of patient infectiousness, disease severity and monitoring treatment response in human and in-vitro laboratory models of disease. We hypothesized that newer techniques would perform as well as solid media culture to quantify mycobacterial burden in laboratory specimens. Methods: We compared the turn-around-time, detection-threshold, dynamic range, reproducibility, relative discriminative ability, of 4 mycobacterial load determination techniques: automat