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comparison of microarray platforms for measuring differential microrna expression in paired normalcancer colon tissues比较测量微分微rna表达的微阵列平台配对normalcancer结肠组织
Comparison of Microarray Platforms for Measuring Differential MicroRNA Expression in Paired Normal/ Cancer Colon Tissues 1,2 1 2 1 3 Maurizio Callari , Matteo Dugo , Valeria Musella , Edoardo Marchesi , Giovanna Chiorino , Maurizia 3 5 2 1,4 1 Mello Grand , Marco Alessandro Pierotti , Maria Grazia Daidone , Silvana Canevari *, Loris De Cecco 1 Functional Genomics Core Facility, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy, 2 Unit of Biomarkers, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy, 3 Cancer Genomic Laboratory, Edo ed Elvo Tempia Foundation, Biella, Italy, 4 Unit of Molecular Therapies, Department of Experimental Oncology and Molecular Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy, 5 Scientific Directorate, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy Abstract Background: Microarray technology applied to microRNA (miRNA) profiling is a promising tool in many research fields; nevertheless, independent studies characterizing the same pathology have often reported poorly overlapping results. miRNA analysis methods have only recently been systematically compared but only in few cases using clinical samples. Methodology/Principal Findings: We investigated the inter-platform reproducibility of four miRNA microarray platforms (Agilent, Exiqon, Illumina, and Miltenyi), comparing nine paired tumor/normal colon tissues. The most concordant and selected discordant miRNAs were further studied by quantitative RT-PCR. Globally, a poor o
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