commensal bacteria and expression of two major intestinal chemokines, teckccl25 and mecccl28, and their receptors共生的细菌和两个主要的肠道趋化因子的表达,teckccl25 mecccl28,及其受体.pdfVIP

Commensal Bacteria and Expression of Two Major Intestinal Chemokines, TECK/CCL25 and MEC/CCL28, and Their Receptors 1 1 2 2 1 2 3 Franc¸ois Meurens *, Mustapha Berri , Richard H. Siggers , Benjamin P. Willing , Henri Salmon , Andrew G. Van Kessel , Volker Gerdts ´ ´ 1 Lymphocyte et Immunite des Muqueuses, UR 1282, Infectiologie Animale et Sante Publique, Institut National de la Recherche Agronomique, Nouzilly, France, 2 Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, Saskatchewan, Canada, 3 Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan, Canada Background. CCL25/TECK and CCL28/MEC are CC chemokines primarily expressed in thymic dendritic cells and mucosal epithelial cells. Their receptors, CCR9 and CCR10, are mainly expressed on T and B lymphocytes. In human, mouse, pig and sheep CCL25 and CCL28 play an important role in the segregation and the compartmentalization of the mucosal immune system. As evidenced by early comparisons of germ-free and conventional animals, the intestinal bacterial microflora has a marked effect on host intestinal immune functions. However, little is known about the impact of bacterial colonization on constitutive and induced chemokine expressions as well as on the generation of anti-inflammatory mechanisms. Methodology/Principal Findings. Therefore, we decided to focus by qPCR on the mRNA expression of two main gut chemokines, CCL25 and CCL28, their receptors CCR9 and CCR10, the Tregs marker Foxp3 and anti-inflammatory cytokines TGF-