color photographic index of fall chinook salmon embryonic development and accumulated thermal units彩色摄影指数下降大鳞大麻哈鱼胚胎发育和累积热量单位.pdfVIP

Color Photographic Index of Fall Chinook Salmon Embryonic Development and Accumulated Thermal Units ¤ James W. Boyd* , Eric W. Oldenburg, Geoffrey A. McMichael Ecology Group, Battelle, Richland, Washington, United States of America Abstract Background: Knowledge of the relationship between accumulated thermal units and developmental stages of Chinook salmon embryos can be used to determine the approximate date of egg fertilization in natural redds, thus providing insight into oviposition timing of wild salmonids. However, few studies have documented time to different developmental stages of embryonic Chinook salmon and no reference color photographs are available. The objectives of this study were to construct an index relating developmental stages of hatchery-reared fall Chinook salmon embryos to time and temperature (e.g., degree days) and provide high-quality color photographs of each identified developmental stage. Methodology/Principal Findings: Fall Chinook salmon eggs were fertilized in a hatchery environment and sampled approximately every 72 h post-fertilization until 50% hatch. Known embryonic developmental features described for sockeye salmon were used to describe development of Chinook salmon embryos. A thermal sums model was used to describe the relationship between embryonic development rate and water temperature. Mean water temperature was 8.0uC (range; 3.9–11.7uC) during the study period. Nineteen stages of embryonic development were identified for fall Chinook salmon; two stages in the cleavage phase, one stage in the gastrulation phase, and sixteen stages in the organogenesis phase. The thermal sums model used in this study provided similar estimates of fall Chinook salmon embryoni