combination of oxyanion gln114 mutation and medium engineering to influence the enantioselectivity of thermophilic lipase from geobacillus zalihae结合oxyanion gln114突变和介质工程影响高温脂肪酶的选择性geobacillus zalihae.pdfVIP
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combination of oxyanion gln114 mutation and medium engineering to influence the enantioselectivity of thermophilic lipase from geobacillus zalihae结合oxyanion gln114突变和介质工程影响高温脂肪酶的选择性geobacillus zalihae
Int. J. Mol. Sci. 2012, 13, 11666-11680; doi:10.3390/ijms130911666 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Combination of Oxyanion Gln114 Mutation and Medium Engineering to Influence the Enantioselectivity of Thermophilic Lipase from Geobacillus zalihae Roswanira Abdul Wahab 1,2,*, Mahiran Basri 1,*, Mohd Basyaruddin Abdul Rahman 1, Raja Noor Zaliha Raja Abdul Rahman 3,4, Abu Bakar Salleh 3,4 and Thean Chor Leow 3,4 1 Enzyme and Microbial Technology Group, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia; E-Mail: basya@.my 2 Faculty of Science, Universiti Teknologi Malaysia, Skudai, Johor 81310, Malaysia 3 Enzyme and Microbial Technology Group, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia; E-Mails: rnzaliha@.my (R.N.Z.R.A.R.); abubakar@.my (A.B.S.); adamleow@.my (T.C.L.) 4 Institute of Bioscience, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia * Authors to whom correspondence should be addressed; E-Mails: roswanira@kimia.fs.utm.my (R.A.W.); mahiran@.my (M.B.); Tel.: +607-553-4148 (R.A.W.); +603-8946-8954 (M.B.); Fax: +607-556-6162 (R.A.W.); +603-8943-2508 (M.B.). Received: 25 May 2012; in revised form: 10 August 2012 / Accepted: 20 August 2012 / Published: 17 September 2012 Abstract: The substitution of the oxyanion Q114 with Met and Leu was carried out to investigate
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