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blood peptidome-degradome profile of breast cancer血peptidome-degradome的乳腺癌
Blood Peptidome-Degradome Profile of Breast Cancer 1 ´2 1 2 3 1 Yufeng Shen *, Nikola Tolic , Tao Liu , Rui Zhao , Brianne O. Petritis , Marina A. Gritsenko , David G. 1 1 2 4 1,2 Camp , Ronald J. Moore , Samuel O. Purvine , Francisco J. Esteva , Richard D. Smith * 1 Biological Science Division, Pacific Northwest National Laboratory, Richland, Washington, United States of America, 2 Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, United States of America, 3 Biological Design Interdisciplinary Graduate Degree Program, Arizona State University, Tempe, Arizona, United States of America, 4 Departments of Breast Medical Oncology and Molecular and Cellular Oncology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas, United States of America Abstract Background: Cancer invasion and metastasis are closely associated with activities within the degradome; however, little is known about whether these activities can be detected in the blood of cancer patients. Methodology and Principal Findings: The peptidome-degradome profiles of pooled blood plasma sampled from 15 breast cancer patients (BCP) and age, race, and menopausal status matched control healthy persons (HP) were globally characterized using advanced comprehensive separations combined with tandem Fourier transform mass spectrometry and new data analysis approaches that facilitated top-down peptidomic analysis. The BCP pool displayed 71 degradome protein substrates that encompassed 839
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