cohesin interaction with centromeric minichromosomes shows a multi-complex rod-shaped structure与着丝粒minichromosomes cohesin交互显示multi-complex杆状结构.pdfVIP

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cohesin interaction with centromeric minichromosomes shows a multi-complex rod-shaped structure与着丝粒minichromosomes cohesin交互显示multi-complex杆状结构.pdf

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cohesin interaction with centromeric minichromosomes shows a multi-complex rod-shaped structure与着丝粒minichromosomes cohesin交互显示multi-complex杆状结构

Cohesin Interaction with Centromeric Minichromosomes Shows a Multi-Complex Rod-Shaped Structure 1¤ 2 1,3 1,4{ Alexandra Surcel , Douglas Koshland , Hong Ma *, Robert T. Simpson 1The Intercollege Graduate Program in Cell and Developmental Biology, The Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania, United States of America, 2 Howard Hughes Medical Institute, Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland, United States of America, 3 Department of Biology, The Pennsylvania State University, University Park, Pennsylvania, United States of America, 4 Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania, United States of America Abstract Cohesin is the protein complex responsible for maintaining sister chromatid cohesion. Cohesin interacts with centromeres and specific loci along chromosome arms known as Chromosome Attachment Regions (CARs). The cohesin holocomplex contains four subunits. Two of them, Smc1p (Structural maintenance of chromosome 1 protein) and Smc3p, are long coiled- coil proteins, which heterodimerize with each other at one end. They are joined together at the other end by a third subunit, Scc1p, which also binds to the fourth subunit, Scc3p. How cohesin interacts with chromosomes is not known, although several models have been proposed, in part on the basis of in vitro assembly of purified cohesin proteins. To be able to observe in vivo cohesin-chromatin interactions, we have modified a Minichromosome Affinity Purification (MAP) method to isolate a CAR-containing centromeric minichromosome attached to in vivo assembled cohesin.