biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific gtpase rab11b生物起源的内膜复杂取决于膜泡运输的蜂窝状的特定gtpase rab11b.pdfVIP

Biogenesis of the Inner Membrane Complex Is Dependent on Vesicular Transport by the Alveolate Specific GTPase Rab11B 1 . 2. 3 4 Carolina Agop-Nersesian * , Saskia Egarter , Gordon Langsley , Bernardo J. Foth , David J. P. 5 2 Ferguson , Markus Meissner * 1 Department of Infectiology, Parasitology, University Hospital Heidelberg, Heidelberg, Germany, 2 Division of Infection Immunity and Wellcome Centre for Parasitology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, United Kingdom, 3 Laboratoire de Biologie Cellulaire Comparative des Apicomplexes, Institut ´ ´ ˆ Cochin, Inserm, U567, CNRS, UMR 8104, Faculte de Medecine Paris V – Hopital Cochin, Paris, France, 4 School of Biological Sciences, Nanyang Technological University, Singapore, Republic of Singapore, 5 Nuffield Department of Clinical Laboratory Science, Oxford University, Oxford, United Kingdom Abstract Apicomplexan parasites belong to a recently recognised group of protozoa referred to as Alveolata. These protists contain membranous sacs (alveoli) beneath the plasma membrane, termed the Inner Membrane Complex (IMC) in the case of Apicomplexa. During parasite replication the IMC is formed de novo within the mother cell in a process described as internal budding. We hypothesized that an alveolate specific factor is involved in the specific transport of vesicles from the Golgi to the IMC and identified the small GTPase Rab11B as an alveolate specific Rab-GTPase that localises to the growing end of the IMC during replication of Toxoplasma gondii. Conditional interference with Rab11B function leads to a profound defect in IMC biogenesis, indicatin