a dna polymerase α accessory protein, mcl1, is required for propagation of centromere structures in fission yeastdna聚合酶α辅助蛋白质,mcl1,需要传播裂殖酵母的着丝粒结构.pdfVIP

A DNA Polymerase a Accessory Protein, Mcl1, Is Required for Propagation of Centromere Structures in Fission Yeast 1¤a 1 2 3¤b 3 Toyoaki Natsume , Yasuhiro Tsutsui *, Takashi Sutani , Elaine M. Dunleavy , Alison L. Pidoux , 4 2 3 1 Hiroshi Iwasaki , Katsuhiko Shirahige , Robin C. Allshire , Fumiaki Yamao 1 Division of Mutagenesis, National Institute of Genetics, Mishima, Shizuoka, Japan, 2 Laboratory of Genome Structure Function, Center for Biological Resources and Informatics, Tokyo Institute of Technology, Yokohama, Kanagawa, Japan, 3 Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, School of Biological Sciences, The University of Edinburgh, Edinburgh, Scotland, United Kingdom, 4 Division of Molecular and Cellular Biology, International Graduate School of Arts and Sciences, Yokohama City University, Yokohama, Kanagawa, Japan Abstract Specialized chromatin exists at centromeres and must be precisely transmitted during DNA replication. The mechanisms involved in the propagation of these structures remain elusive. Fission yeast centromeres are composed of two chromatin domains: the central CENP-ACnp1 kinetochore domain and flanking heterochromatin domains. Here we show that fission yeast Mcl1, a DNA polymerase a (Pola) accessory protein, is critical for maintenance of centromeric chromatin. In a screen for mutants that alleviate both central domain and outer repeat silencing, we isolated several cos mutants, of which cos1 is allelic to mcl1. The mcl1-101 mutation causes reduced CENP-ACnp1 in the central domain and an aberrant increase in histone acetylation in