vernalization-repression of arabidopsis flc requires promoter sequences but not antisense transcripts拟南芥的vernalization-repression方法需要但不是反义转录启动子序列.pdfVIP

Vernalization-Repression of Arabidopsis FLC Requires Promoter Sequences but Not Antisense Transcripts ¤ Chris A. Helliwell*, Masumi Robertson, E. Jean Finnegan, Diana M. Buzas , Elizabeth S. Dennis CSIRO Plant Industry, Canberra, Australia Abstract The repression of Arabidopsis FLC expression by vernalization (extended cold) has become a model for understanding polycomb-associated epigenetic regulation in plants. Antisense and sense non-coding RNAs have been respectively implicated in initiation and maintenance of FLC repression by vernalization. We show that the promoter and first exon of the FLC gene are sufficient to initiate repression during vernalization; this initial repression of FLC does not require antisense transcription. Long-term maintenance of FLC repression requires additional regions of the gene body, including those encoding sense non-coding transcripts. Citation: Helliwell CA, Robertson M, Finnegan EJ, Buzas DM, Dennis ES (2011) Vernalization-Repression of Arabidopsis FLC Requires Promoter Sequences but Not Antisense Transcripts. PLoS ONE 6(6): e21513. doi:10.1371/journal.pone.0021513 ´ Editor: Miguel A. Blazquez, Instituto de Biologıa Molecular y Celular de Plantas, Spain Received May 11, 2011; Accepted May 31, 2011; Published June 21, 2011 Copyright: 2011 Helliwell et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: These authors have no support or funding to report. Competing Interests: The authors have declared t