variation in stability of endogenous reference genes in fallopian tubes and endometrium from healthy and ectopic pregnant women内生稳定性的变化从健康和参考基因在输卵管和子宫内膜异位孕妇.pdfVIP

Int. J. Mol. Sci. 2012, 13, 2810-2826; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Variation in Stability of Endogenous Reference Genes in Fallopian Tubes and Endometrium from Healthy and Ectopic Pregnant Women 1 2 1 1 Alpha K. Gebeh , Emma L. Marczylo , Akwasi A. Amoako , Jonathon M. Willets and Justin C. Konje 1,* 1 Endocannabinoid Research Group, Reproductive Science Section, Department of Cancer Studies and Molecular Medicine, University of Leicester, Leicester, LE2 7LX, UK; E-Mails: ag271@le.ac.uk (A.K.G.); aaa57@le.ac.uk (A.A.A.); jmw23@le.ac.uk (J.M.W.) 2 Systems Toxicology, MRC Toxicology Unit, Hodgkin Building, University of Leicester, Lancaster Road, Leicester, LE1 9HN, UK; E-Mail: elt14@le.ac.uk * Author to whom correspondence should be addressed; E-Mail: jck4@le.ac.uk; Tel.: +44-116-252-5826; Fax: +44-116-252-3162. Received: 5 January 2012; in revised form: 10 February 2012 / Accepted: 27 February 2012 / Published: 2 March 2012 Abstract: RT-qPCR is commonly employed in gene expression studies in ectopic pregnancy. Most use RN18S1, β-actin or GAPDH as internal controls without validation of their suitability as reference genes. A systematic study of the suitability of endogenous reference genes for gene expression stu