the expression of selenium-binding protein 1 is decreased in uterine leiomyomaselenium-binding的表达蛋白1是减少子宫平滑肌瘤.pdfVIP

Zhang et al. Diagnostic Pathology 2010, 5:80 /content/5/1/80 RESEARCH Open Access The expression of selenium-binding protein 1 is decreased in uterine leiomyoma * Peng Zhang, Cunxian Zhang , Xudong Wang, Fang Liu, C James Sung, M Ruhul Quddus, W Dwayne Lawrence Abstract Background: Selenium has been shown to inhibit cancer development and growth through the mediation of selenium-binding proteins. Decreased expression of selenium-binding protein 1 has been reported in cancers of the prostate, stomach, colon, and lungs. No information, however, is available concerning the roles of selenium- binding protein 1 in uterine leiomyoma. Methods: Using Western Blot analysis and immunohistochemistry, we examined the expression of selenium- binding protein 1 in uterine leiomyoma and normal myometrium in 20 patients who had undergone hysterectomy for uterine leiomyoma. Results and Discussion: The patient age ranged from 34 to 58 years with a mean of 44.3 years. Proliferative endometrium was seen in 8 patients, secretory endometrium in 7 patients, and atrophic endometrium in 5 patients. Two patients showed solitary leiomyoma, and eighteen patients revealed 2 to 5 tumors. Tumor size ranged from 1 to 15.5 cm with a mean of 4.3 cm. Both Western Blot analysis and immunohistochemistry showed a significant lower level of selenium-binding protein 1 in leiomyoma than in normal myometrium. Larger tumors had a tendency to show a lower level of selenium-binding protein 1 than smaller ones, but the difference did not reach a statistical significance. The expression of selenium-binding protein 1 was the same among patients with proliferative, secretory, and atrophic endometrium in either leiomyoma or normal myometrium. Also, we did not find a difference of selenium-binding protein 1 level betwe