tasting soil fungal diversity with earth tongues phylogenetic test of saté alignments for environmental its data品尝土壤真菌多样性与地球舌头满足校准的系统测试环境的数据.pdfVIP

Tasting Soil Fungal Diversity with Earth Tongues: ´ Phylogenetic Test of SATe Alignments for Environmental ITS Data 1 2,3 1 4 5 Zheng Wang *, R. Henrik Nilsson , Francesc Lopez-Giraldez , Wen-ying Zhuang , Yu-cheng Dai , Peter R. Johnston6, Jeffrey P. Townsend1 1 Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut, United States of America, 2 Department of Plant and Environmental ¨ ¨ Sciences, Goteborg University, Goteborg, Sweden, 3 Department of Botany, Institute of Ecology and Earth Sciences, University of Tartu, Tartu, Estonia, 4 Institute of Microbiology, Chinese Academy of Science, Beijing, China, 5 Institute of Microbiology, Beijing Forestry University, Beijing, China, 6 Herbarium PDD, Landcare Research, Auckland, New Zealand Abstract An abundance of novel fungal lineages have been indicated by DNA sequencing of the nuclear ribosomal ITS region from environmental samples such as soil and wood. Although phylogenetic analysis of these novel lineages is a key component of unveiling the structure and diversity of complex communities, such analyses are rare for environmental ITS data due to the difficulties of aligning this locus across significantly divergent taxa. One potential approach to this issue is simultaneous alignment and tree estimation. We targeted divergent ITS sequences of the earth tongue fungi (Geoglossomycetes), a basal ´ class in the Ascomycota, to assess the performance of SATe, recent software that combines progressive alignment and tree