synthesis and photophysical study of 2′-deoxyuridines labeled with fluorene derivatives的合成及光物理研究2u2032-deoxyuridines标以芴衍生物.pdfVIP

Molecules 2012, 17, 12061-12071; doi:10.3390/molecules171012061 OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Synthesis and Photophysical Study of 2′-Deoxyuridines Labeled with Fluorene Derivatives Hyun Yi Cho 1, Sang Keun Woo 2,* and Gil Tae Hwang 1,* 1 Department of Chemistry and Green-Nano Materials Research Center, Kyungpook National University, Daegu 702-701, Korea; E-Mail: whgusdl840@ 2 Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, 75 Nowon-gil, Seoul 139-706, Korea * Authors to whom correspondence should be addressed; E-Mails: skwoo@kcch.re.kr (S.K.W.); giltae@knu.ac.kr (G.T.H.); Tel.: +82-2-970-1659 (S.K.W.); Fax: +82-2-970-1341 (S.K.W.); Tel.: +82-53-950-5331 (G.T.H.); Fax: +82-53-950-6330 (G.T.H.). Received: 14 September 2012; in revised form: 3 October 2012 / Accepted: 10 October 2012 / Published: 15 October 2012 Abstract: We examined microenvironment-sensitive fluorescent 2′-deoxyuridines labeled with fluorene derivatives that exhibited solvent-dependent photophysical properties. The high sensitivity of the fluorescence shift and the nucleoside intensity dependence on solvent polarity provided information useful for estimating the polarity of the environment surrounding the fluorescent nucleoside. Keywords: nucleosides; fluorene; fluorescence; dibenzofuran; dibenzothiophene 1. Introduction Fluorescent nucleosides which are structurally noninvasive, forming stable Watson-Crick base pairs, and sensiti