survival of influenza a(h1n1) on materials found in households implications for infection control生存的甲型流感(h1n1)材料中发现家庭对感染控制的影响.pdfVIP

Survival of Influenza A(H1N1) on Materials Found in Households: Implications for Infection Control 1 2 1 2 2 Jane S. Greatorex , Paul Digard , Martin D. Curran , Robert Moynihan , Harrison Wensley , Tim 1 1 1 3 3,4 Wreghitt , Harsha Varsani , Fayna Garcia , Joanne Enstone , Jonathan S. Nguyen-Van-Tam * 1 Health Protection Agency, Addenbrookes Hospital, Cambridge, United Kingdom, 2 Division of Virology, Department of Pathology, University of Cambridge, Cambridge, United Kingdom, 3 Division of Epidemiology and Public Health, University of Nottingham, Nottingham, United Kingdom, 4 Health Protection Agency, East Midlands, Nottingham, United Kingdom Abstract Background: The majority of influenza transmission occurs in homes, schools and workplaces, where many frequently touched communal items are situated. However the importance of transmission via fomites is unclear since few data exist on the survival of virus on commonly touched surfaces. We therefore measured the viability over time of two H1N1 influenza strains applied to a variety of materials commonly found in households and workplaces. Methodology and Principal Findings: Influenza A/PuertoRico/8/34 (PR8) or A/Cambridge/AHO4/2009 (pandemic H1N1) viruses were inoculated onto a wide range of surfaces used in home and work environments, then sampled at set times following incubation at stabilised temperature and humidity. Virus genome was measured by RT-PCR; plaque assay (for PR8) or fluorescent focus formation (for pandemic H1N1) was used to assess the surviv